Effects of chilled storage on fish freshness using computer vision and artificial neural network modeling

Keywords: color analysis, fish meat, eye color, quality control, storage time

Abstract

The present study investigates the impact of refrigeration storage on the freshness and shelf life of European sea bass (Dicentrarchus labrax). This investigation utilises computer vision systems and artificial neural networks (ANNs) to analyse the dynamics of the process. A non-destructive assessment approach was established by analysing the eye colour characteristics (RGB, Lab*, and HSI values) of fish stored at +4 °C for 15 days, with sampling occurring every three days. There were considerable changes in the colour range throughout the time, particularly a reduction of brightness (L*), which can be one indicator of the progressive deterioration of the fish›s freshness. The neural network  multilayer perceptron was  optimised  with  20  neurons  in  the  hidden  layer  and demonstrated a high correlation coefficient (R² = 0.98) between predicted and experimental shelf life values. The data indicates that the values of rack life, which were initially determined to be cautious, exhibited a high degree of correlation with the estimated values. The R2 value was determined to be 0.98. The technique offers a rapid and reliable non-destructive method for determining the freshness of fish, with potential applications in relevant areas such as quality control and natural security examination for aquaculture products. The present study investigates the impact of refrigeration storage on the freshness and shelf life of European sea bass (Dicentrarchus labrax). This investigation utilises computer vision systems and artificial neural networks (ANNs) to analyse the dynamics of the process. A non-destructive assessment approach was established by analysing the eye colour characteristics (RGB, Lab*, and HSI values) of fish stored at +4 °C for 15 days, with sampling occurring every three days. There were considerable changes in the colour range throughout the time, particularly a reduction of brightness (L*), which can be one indicator of the progressive deterioration of the fish›s freshness. The neural network  multilayer perceptron was  optimised  with  20  neurons  in  the  hidden  layer  and demonstrated a high correlation coefficient (R² = 0.98) between predicted and experimental shelf life values. The data indicates that the values of rack life, which were initially determined to be cautious, exhibited a high degree of correlation with the estimated values. The R2 value was determined to be 0.98. The technique offers a rapid and reliable non-destructive method for determining the freshness of fish, with potential applications in relevant areas such as quality control and natural security examination for aquaculture products.

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References

Erdağ M, Ayvaz Z. The use of color to determine fish freshness: European seabass (Dicentrarchus labrax). J. Aquat. Food Prod. Technol. [Internet]. 2021; 30(7):847– 867. doi: https://doi.org/pwvr

Mokrani D, Oumouna M, Cuesta A. Fish farming conditions affect to European sea bass (Dicentrarchus labrax L.) quality and shelf life during storage in ice. Aquaculture. [Internet]. 2018; 490:120–124. doi: https://doi.org/gdcqwq

Wu X, Zhang Q, Wang Z, Wang Z, Yan H, Zhu L, Chang J. Nondestructive freshness prediction of large yellow croaker (Pseudosciaena crocea) using computer vision and machine learning techniques based on pupil color. J. Food Sci. [Internet]. 2024; 89(12):9392–9406. doi: https://doi.org/pwvt

Yi Z, Xie J. Assessment of spoilage potential and amino acids deamination & decarboxylation activities of Shewanella putrefaciens in bigeye tuna (Thunnus obesus). LWT. [Internet]. 2022; 156:113016. doi: https://doi.org/gq2wg7

Cheng JH, Sun DW. Rapid and non-invasive detection of fish microbial spoilage by visible and near infrared hyperspectral imaging and multivariate analysis. LWT Food Sci. Technol. [Internet]. 2015; 62(2):1060–1068. doi: https://doi.org/gq33j4

Zhang Y, Qin N, Luo Y, Shen H. Effects of different concentrations of salt and sugar on biogenic amines and quality changes of carp (Cyprinus carpio) during chilled storage. J. Sci. Food Agric. [Internet]. 2015; 95(6):1157– 1162. doi: https://doi.org/gj89h7

Chun HN, Kim B, Shin HS. Evaluation of a freshness indicator for quality of fish products during storage. Food Sci. Biotechnol. [Internet]. 2014; 23:1719–1725. doi: https://doi.org/f6nkz3

Liao Q, Wei C, Li Y, Gou L, Ouyang H. Developing a machine vision system equipped with UV light to predict fish freshness based on fish-surface color. Food Nutr. Sci. [Internet]. 2021; 12(3):239–248. doi: https://doi.org/gpbc4m

Sigurgisladottir S, Hafsteinsson H, Jonsson A, Lie Ø, Nortvedt R, Thomassen M, Torrissen O. Textural properties of raw salmon fillets as related to sampling method. J. Food Sci. [Internet]. 1999; 64(1):99–104. doi: https://doi.org/dmntwv

Prabhakar PK, Vatsa S, Srivastav PP, Pathak SS. A comprehensive review on freshness of fish and assessment: Analytical methods and recent innovations. Food Res. Int. [Internet]. 2020; 133:109157. doi: https://doi.org/mpdk

Wu L, Pu H, Sun DW. Novel techniques for evaluating freshness quality attributes of fish: A review of recent developments. Trends Food Sci. Technol. [Internet]. 2019; 83:259–273. doi: https://doi.org/gjvdn3

Gümüş B, Gümüş E, Balaban MO. Color of rainbow trout (Oncorhynchus mykiss) fillets by image and sensory analysis, and correlation with SalmoFan numbers. J. Food Sci. [Internet]. 2023; 88(1):430–446. doi: https://doi.org/pwv2

Korel F, Luzuriaga DA, Balaban MÖ. Objective quality assessment of raw tilapia (Oreochromis niloticus) fillets using electronic nose and machine vision. J. Food Sci. [Internet]. 2001; 66(7):1018–1024. doi: https://doi.org/cv9zq7

Cengizler C. Fish spoilage classification based on color distribution analysis of eye images. Mar. Sci. Technol. Bull. [Internet]. 2023; 12(1):63–69. doi: https://doi.org/pwv4

Yasin ET, Ozkan IA, Koklu M. Detection of fish freshness using artificial intelligence methods. Eur. Food Res. Technol. [Internet]. 2023; 249(8):1979–1990. doi: https://doi.org/pwv5

Huang X, Xu H, Wu L, Dai H, Yao L, Han F. A data fusion detection method for fish freshness based on computer vision and near-infrared spectroscopy. Anal. Methods. [Internet]. 2016; 8(14):2929–2935. doi: https://doi.org/gjvdpb

Gümüş B, Balaban MO. Prediction of the weight of aquacultured rainbow trout (Oncorhynchus mykiss) by image analysis. J. Aquat. Food Prod. Technol. [Internet]. 2010; 19(3–4):227–237. doi: https://doi.org/cgs3pv

Misimi E, Erikson U, Digre H, Skavhaug A, Mathiassen JR. Computer vision-based evaluation of pre- and postrigor changes in size and shape of Atlantic cod (Gadus morhua) and Atlantic salmon (Salmo salar) fillets during rigor mortis and ice storage: effects of perimortem handling stress. J. Food Sci. [Internet]. 2008; 73(2):E57–E68. doi: https://doi.org/b48bbd

Huang Y, Kangas LJ, Rasco BA. Applications of artificial neural networks (ANNs) in food science. Crit. Rev. Food Sci. Nutr. [Internet]. 2007; 47(2):113–126. doi: https://doi.org/bffx3k

Gonzalez-Fernandez I, Iglesias-Otero MA, Esteki M, Moldes OA, Mejuto JC, Simal-Gandara J. A critical review on the use of artificial neural networks in olive oil production, characterization and authentication. Crit. Rev. Food Sci. Nutr. [Internet]. 2019; 59(12):1913–1926. doi: https://doi.org/ggb42s

Lakehal S, Lakehal B, Chadi H, Bennoune O, Ayachi A. Effects on beef microstructure using fractal dimension and ANN modelling. J. Hellenic. Vet. Med. Soc. [Internet]. 2024; 75(4):8281–8290. doi: https://doi.org/pwv6

Lalabadi HM, Sadeghi M, Mireei SA. Fish freshness categorization from eyes and gills color features using multi-class artificial neural network and support vector machines. Aquacult. Eng. [Internet]. 2020; 90:102076. doi: https://doi.org/kgdk

Zheng Y, Zhang Q, Wang X, Guo Q. Classifying the freshness of large yellow croaker (Larimichthys crocea) at 12-and 24-hour intervals using computer vision technique and convolutional neural network. Smart Agric. Technol. [Internet]. 2025; 10:100767. doi: https://doi.org/pwv7

Lakehal S, Lakehal B. Storage time prediction of frozen meat using artificial neural network modeling with color values. Rev. Cient. FCVLUZ. [Internet]. 2023; 33(2):1–6. doi: https://doi.org/pwv8

Lakehal B, Dibi Z, Lakhdar N, Dendouga A. Electrical equivalent model of intermediate band solar cell using PSpice. Sadhana. [Internet]. 2015; 40:1473–1479. doi: https://doi.org/kgdn

Dowlati M, Mohtasebi SS, Omid M, Razavi SH, Jamzad M, De La Guardia M. Freshness assessment of gilthead sea bream (Sparus aurata) by machine vision based on gill and eye color changes. J. Food Eng. [Internet]. 2013; 119(2):277–287. doi: https://doi.org/pwv9

Masniyom P. Deterioration and shelf-life extension of fish and fishery products by modified atmosphere packaging. Songklanakarin J. Sci. Technol. [Internet]. 2011 [cited Feb 25 2025]; 33(2):181–192. Available in: https://goo.su/XjfL

Stoknes IS, Økland HM, Falch E, Synnes M. Fatty acid and lipid class composition in eyes and brain from teleosts and elasmobranchs. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. [Internet]. 2004; 138(2):183–191. doi: https://doi.org/bcb45h

Jia Z, Li M, Shi C, Zhang J, Yang X. Determination of salmon freshness by computer vision based on eye color. Food Packag. Shelf Life. [Internet]. 2022; 34:100984. doi: https://doi.org/pwwb

Chmiel M, Słowiński M, Dasiewicz K, Florowski T. Use of computer vision system (CVS) for detection of PSE pork meat obtained from m. semimembranosus. LWT Food Sci. Technol. [Internet]. 2016; 65:532–536. doi: https://doi.org/pwwc

Shi C, Qian J, Han S, Fan B, Yang X, Wu X. Developing a machine vision system for simultaneous prediction of freshness indicators based on tilapia (Oreochromis niloticus) pupil and gill color during storage at 4°C. Food Chem. [Internet]. 2018; 243:134–140. doi: https://doi.org/gjvdpk

Zhou B, Elazab A, Bort J, Vergara O, Serret MD, Araus JL. Low-cost assessment of wheat resistance to yellow rust through conventional RGB images. Comput. Electron. Agric. [Internet]. 2015; 116:20–29. doi: https://doi.org/f7pgr6

Cheng HD, Jiang XH, Sun Y, Wang J. Color image segmentation: Advances and prospects. Pattern Recognit. [Internet]. 2001; 34(12):2259–2281. doi: https://doi.org/dgw93m

Dhandra BV, Hegadi R, Hangarge M, Malemath VS. Analysis of abnormality in endoscopic images using combined HSI color space and watershed segmentation. In: 18th International Conference on Pattern Recognition (ICPR’06). Vol. 4. China: IEEE; 2006. p. 695–698. doi: https://doi.org/cxkh5f

Aggabou LK, Lakehal B, Mouda M. An Artificial Neural Network Approach for Construction Project Risk Management. Int. J. Saf. Secur. Eng. [Internet]. 2024; 14(2):553–561. doi: https://doi.org/pwwd

Liu X, Jiang Y, Shen S, Luo Y, Gao L. Comparison of Arrhenius model and artificial neuronal network for the quality prediction of rainbow trout (Oncorhynchus mykiss) fillets during storage at different temperatures. LWT Food Sci. Technol. [Internet]. 2015; 60(1):142–147. doi: https://doi.org/gsrqzm

Rezende-de-Souza JH, de Moraes-Neto VF, Cassol GZ, dos Santos Camelo MC, Savay-da-Silva LK. Use of colorimetric data and artificial neural networks for the determination of freshness in fish. Food Chem. Adv. [Internet]. 2022; 1:100129. doi: https://doi.org/pwwf

Published
2025-07-23
How to Cite
1.
Lakehal S, Lakehal B. Effects of chilled storage on fish freshness using computer vision and artificial neural network modeling. Rev. Cient. FCV-LUZ [Internet]. 2025Jul.23 [cited 2025Aug.18];35(3):7. Available from: https://produccioncientifica.luz.edu.ve/index.php/cientifica/article/view/44129
Section
Food Science and Technology