Identification, characterization, and purification of xylanase PC-01 isolated from Pacet hot spring

  • Mamik Damayanti, Ni Nyoman Purwani Research Center for Bio-Molecule Engineering, Universitas Airlangga, Kampus C-UNAIR, Mulyorejo, Surabaya, 60115, Indonesia
  • Ni Made Dwi Purwadi Departement of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus C-UNAIR, Mulyorejo, Surabaya, 60115, Indonesia
  • Lailatul Fithri Research Center for Bio-Molecule Engineering, Universitas Airlangga, Kampus C-UNAIR, Mulyorejo, Surabaya, 60115, Indonesia
Palabras clave: Endo-Β-Xylanase, Hydrophobic, Interactions, Chromatography

Resumen

This research aims to characterize, identify and purify the xylanolytic enzymes by hydrophobic interaction and anion exchange chromatography via the endo-β-xylanase activity test with the DNS method. The best results obtained from anion-exchange chromatography of third fraction pH 5 with the purity level of 78.25 times higher than crude extract. In conclusion, Characteristics of bacterial isolates PC-01 are rod-shaped bacterial cells, Gram-positive, and have subterminal oval endospores.
Publicado
2019-12-31
Cómo citar
Ni Nyoman Purwani, M. D., Dwi Purwadi, N. M., & Fithri, L. (2019). Identification, characterization, and purification of xylanase PC-01 isolated from Pacet hot spring. Opción, 35, 1074-1091. Recuperado a partir de https://produccioncientifica.luz.edu.ve/index.php/opcion/article/view/30731
Número
Vol. 35 (2019): Edición Especial Nro. 24
Sección
Artículos