Lycopene regulates the formation of calcium oxalate kidney stones by modulating reactive oxygen species(ROS) and NF-κB pathways.:

Liangwen  Ye; Yuhang  Tang; Zhijie  Zhang; Xiangyi  Hou; Wei  Xu; Xianghui  Suo; Li  Zhang

doi:10.54817/IC.v66n2a07

Liangwen Ye The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0003-9780-4688
Yuhang Tang The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0009-6263-0570
Zhijie Zhang The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0006-8535-0748
Xiangyi Hou The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0004-5185-6965
Wei Xu The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0004-0948-5822
Xianghui Suo The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0008-1688-0694
Li Zhang The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing City, Jiangsu province, China. https://orcid.org/0009-0002-2514-6690

DOI: https://doi.org/10.54817/IC.v66n2a07

Keywords: lycopene, renal tubular epithelial cells, oxalic acid, reactive oxygen species, apoptosis

Abstract

This study aims to determine whether lycopene can reduce oxidative stress and inflammatory damage in HK-2 cell cultures induced by calcium oxalate crystallization through the modulation of reactive oxygen species (ROS) and the NF-κB signalling pathway. Cell cultures were divided into four groups: The control group, the Model group (COM + oxalic acid), and two Lycopene intervention groups (COM + oxalic acid + 5/10 μmol/L lycopene). After 24 hours of culture, viability, LDH, oxidative and anti-oxidative parameters, mitochondrial membrane potential, MCP-1, IL -6, apoptosis and related proteins, and activation and expression of NF-κB were determined by adequate methods. When compared to the control group, the model group exhibited decreased cell activity (p<0.001) and GSH and SOD antioxidant capacity (p<0.05), along-side a significant rise in LDH, MDA, and the release of inflammatory mediators MCP-1 and IL -6 (p<0.05). The levels of protein expression for NF-κB, OPN, Bax, Cyt C, and active Caspase-3 were increased (p<0.05), whereas Bcl-2 protein expression significantly diminished (p<0.05). The mitochondrial membrane potential decreased. Lycopene intervention reduced the damage to HK-2 cells (p<0.05), accompanied by decreased levels of LDH, MDA, and inflammatory factors MCP-1 and IL -6 (p<0.05), and increased GSH and SOD antioxidant capacity (p<0.05). The mitochondrial membrane potential was observed to increase. No significant changes were observed in the expression of NF-κB. The expressions of OPN, Bax, Cyt C, and Caspase-3 decreased (p<0.05), whereas the level of Bcl-2 protein expression increased. In conclusion, lycopene decreas- es cellular damage by inhibiting lipid peroxidation induced by calcium oxalate crystals and oxalate, enhancing intracellular antioxidant enzyme activity, modulating ROS and NF-κB inflammatory pathways, improving mitochondrial integrity, and exerting anti-inflammatory effects through the inhibition of the mitochondrial-mediated Bax/Caspase-3 signalling pathway.

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Lycopene regulates the formation of calcium oxalate kidney stones by modulating reactive oxygen species(ROS) and NF-κB pathways.

El licopeno regula la formación de cálculos renales de oxalato cálcico modulando las vías de especies reactivas de oxígeno (ROS) y NF-κB.

Abstract

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