Effects of Lactobacillus rhamnosus GG on some adipokine levels in rats fed Western diet
Abstract
Adipokines regulate energy homeostasis. High-fat Western diet leads to obesity and disrupts adipocyte metabolism. Probiotics such as Lactobacillus rhamnosus GG are effective in obesity management. This study investigates the effect of Lactobacillus rhamnosus GG on serum adipokine levels in rats fed a High-fat Western diet. Thirty 5-6 week old male Wistar albino rats (Rattus norvegicus domestica) were randomly selected and divided into three groups: control (standard chow), W (45% fat), and a WLgroup supplemented orally with Lactobacillus rhamnosus GG in vegetable oil for 16 weeks. At the end of the study, blood samples were collected from rats via cardiac puncture, and serum adipokine levels were measured using ELISA kits. Body weight, naso-anal length, and BMI (body mass index) exhibited statistically significant increases in the W group. In the Lactobacillus rhamnosus GG-supplemented group (WL), body weight and BMI decreased, while naso-anal length increased. SFRP5 and CT1 levels were low, and asprosin and vaspin levels were high in the Wgroup. CT1, vaspin, and asprosin levels increased in the Lactobacillus rhamnosus GG- supplemented group. In conclusion, Lactobacillus rhamnosus GG alleviates obesity, increases naso-anal length, and reduces BMI in rats fed a High-fat Western diet. These results suggest that L. rhamnosus may regulate serum CT1, vaspin, and asprosin levels, but not omentin or SFRP5 in the High-fat Western diet model.
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