Effects of chlorpyrifos on the freshwater mussel Unio elongatulus eucirrus (Bourguignat, 1860), with a focus on neurotoxicity and oxidative stress responses
Abstract
This study set out to investigate the effects of the organophosphate insecticide chlorpyrifos (CPF) on certain biochemical parameters of the freshwater mussel Unio elongatulus eucirrus. Mussels were exposed to varying concentrations of CPF (10, 20, and 40 µg·L-1) for duration of 96 hours. Samples were collected from the mussels at the 24th and 96th hours of the experiment. The results indicated that exposure to CPF concentrations led to a decline in the activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and, accompanied by an increase in malondialdehyde (MDA), and decreased reduced glutathione (GSH) levels in mussels. In conclusion, the present study demonstrates that CPF caused AChE inhibition, formation of oxidative stress, and negative effects on certain antioxidant parameters in the freshwater mussel U. elongatulus eucirrus.
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References
Hanna NS, Shekha YA. Acute toxicity of chlorpyrifos on the freshwater bivalves (Unio Tigridis) and effects on bioindicators. Baghdad Sci. J. [Internet]. 2024; 21(1):53–61. doi: https://doi.org/pgvc
Mishra A, Singh A. Chlorpyrifos effect on vitellogenin, ovarian steroid in adult and nr5a1 expression in fry of the freshwater catfish, Heteropneustes fossilis (Bloch, 1794). Asian J. Biol. Life Sci. [Internet]. 2021; 10(1): 67–75. doi: https://doi.org/gkc5kg
Liu S, Lu J, Li Z. Water quality criteria derivation and ecological risk assessment for organophosphorus pesticides. Chemosphere [Internet]. 2024; 348:140726. doi: https://doi.org/gwnxjw
Gonçalves AM, Rocha CP, Marques JC, Gonçalves FJ. Enzymes as useful biomarkers to assess the response of freshwater communities to pesticide exposure–A review. Ecol. Indic. [Internet]. 2021; 122:107303. doi: https://doi.org/gzh43x
Huang X, Cui H, Duan W. Ecotoxicity of chlorpyrifos to aquatic organisms: A review. Ecotoxicol. Environ. Saf. [Internet]. 2020; 200:110731. doi: https://doi.org/gksnkh
Li X, Bai Y, Bi Y, Wu Q, Xu S. Baicalin suppressed necroptosis and inflammation against chlorpyrifos toxicity; involving in ER stress and oxidative stress in carp gills. Fish Shellfish Immunol. [Internet]. 2023; 139:108883. doi: https://doi.org/pgvd
Fernández B, Campillo JA, Chaves–Pozo E, Bellas J, León VM, Albentosa M. Comparative role of microplastics and microalgae as vectors for chlorpyrifos bioacumulation and related physiological and immune effects in mussels. Sci. Total Environ. [Internet]. 2022; 807(Part 3):150983. doi: https://doi.org/gp96s3
Perić L, Burić P. The effect of copper and chlorpyrifos co– exposure on biomarkers in the marine mussel Mytilus galloprovincialis. Chemosphere [Internet]. 2019; 225:126–134. doi: https://doi.org/gvtmr3
Pala A. The effect of a glyphosate–based herbicide on acetylcholinesterase (AChE) activity, oxidative stress, and antioxidant status in freshwater amphipod: Gammarus pulex (Crustacean). Environ. Sci. Pollut. Res. [Internet]. 2019; 26(36):36869–36877. doi: https://doi.org/gwdtwc
Yonar ME. Chlorpyrifos–induced biochemical changes in Cyprinus carpio: Ameliorative effect of curcumin. Ecotoxicol. Environ. Saf. [Internet]. 2018; 151:49–54. doi: https://doi.org/gc8rkw
Pala A, Serdar O, Mişe–Yonar S, Yonar ME. Ameliorative effect of Fennel (Foeniculum vulgare) essential oil on chlorpyrifos toxicity in Cyprinus carpio. Environ. Sci. Pollut. Res. [Internet]. 2021; 28:890–897. doi: https://doi.org/pgvf
Mişe–Yonar S, Yonar ME, Ural MŞ, Pala A. Effect of chlorpyrifos on some biochemical changes in Cyprinus carpio: the protective effect of ellagic acid. Drug Chem. Toxicol. [Internet]. 2022; 45(6):2860–2865. doi: https://doi.org/pgvg
Salerno J, Gillis PL, Khan H, Burton E, Deeth LE, Bennett CJ, Sibley PK, Prosser RS. Sensitivity of larval and juvenile freshwater mussels (Unionidae) to ammonia, chloride, copper, potassium, and selected binary chemical mixtures. Environ. Pollut. [Internet]. 2020; 256:113398. doi: https://doi.org/gwg24w
Said RM, Nassar SE. Mortality, energy reserves, and oxidative stress responses of three native freshwater mussels to temperature as an indicator of potential impacts of climate change: A laboratory experimental approach. J. Therm. Biol. [Internet]. 2022; 104: 103154. doi: https://doi.org/pgvj
Şahin AG, Karatepe M. Vitamins A, E, C, β–carotene contents and MDA level of freshwater mussel, (Unio elongatulus eucirrus Bourguignat 1860) in the Karakaya Dam Lake Ege. J. Fish. Aquat. Sci. [Internet]. 2022; 39(2):120–124. doi: https://doi.org/pgvk
Sangsawang A, Kovitvadhi U, Clearwater S J, Kovitvadhi S, Satapornvanit K, Thompson K. Acute toxicity of chlorpyrifos and carbosulfan to glochidia of the freshwater mussel Hyriopsis bialata Simpson, 1900. Environ. Sci. Pollut. Res. [Internet]. 2017; 24:21361–21374. doi: https://doi.org/gbxrgm
Yancheva V, Mollov I, Georgieva E, Stoyanova S, Tsvetanova V, Velcheva I. Ex situ effects of chlorpyrifos on the lysosomal membrane stability and respiration rate in Zebra mussel Dreissena polymorpha (Pallas, 1771). Acta Zool. Bulg. [Internet]. 2017 [cited 26 Nov 2024]; Suppl. 8:85–90. Available in: https://goo.su/eVIRH
Ellman GL, Courtney KD, Andres Jr V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. [Internet]. 1961; 7(2):88–95. doi: https://doi.org/fwdkkz
Lowry OH, Rosebrough N J, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. [Internet]. 1951[cited 26 Nov 2024]; 193(1):265–275. Available in: https://goo.su/nyVwa
Das S, Jana BB. Oxygen uptake and filtration rate as animal health biomarker in Lamellidens marginalis (Lamarck). Indian J. Exp. Biol. [Internet]. 2003 [cited 22 Oct. 2024]; 41:1306– 1310. Available in: https://goo.su/I9SFLGF
Doran WJ, Cope WG, Rada RG, Sandheinrich MB. Acetylcholinesterase inhibition in the threeridge mussel (Amblema plicata) by chlorpyrifos: implications for biomonitoring Ecotoxicol. Environ. Saf. [Internet]. 2001; 49(1):91–98. doi: https://doi.org/fq62p8
Stalin A, Gokula V, Amanullah B. Effect of chlorpyrifos on biochemical changes in freshwater mussel Lamellidens marginalis. Int. J. Appl. Res. [Internet]. 2017 [cited Oct 12 2024]; 3(8):157–159. Available in: https://goo.su/qQK6qNZ
Marnett LJ. Oxy radicals, lipid peroxidation and DNA damage. Toxicol. [Internet]. 2002; 181–182:219–222. doi: https://doi.org/fdjh7n
Ma J, Zhu P, Wang W, Zhang X, Wang P, Sultan Y, Li Y, Ding W, Li, X. Environmental impacts of chlorpyrifos: Transgenerational toxic effects on aquatic organisms cannot be ignored. Sci. Total Environ. [Internet]. 2023; 905:167311. doi: https://doi.org/gtt4sz
Al–Fanharaw, AA, Rabee AM, Al–Mamoori AM. Multi– biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels Unio tigridis and snails Viviparous bengalensis. Hum. Ecol. Risk Assess. [Internet]. 2019; 25(5):1137–1156. doi: https://doi.org/gv33bz
Sharbidre AA, Metkari V, Patode P. Effect of methyl parathion and chlorpyrifos on certain biomarkers in various tissues of guppy fish, Poecilia reticulata. Pestic. Biochem. Physiol. [Internet]. 2011; 101(2):132–141 .doi: https://doi.org/dh37wk
Köprücü K, Yonar SM, Şeker E. Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus. Fish. Sci. [Internet]. 2010; 76:1007–1013. doi: https://doi.org/cgbb4s
Khazri A, Sellami B, Hanachi A, Dellali M, Eljarrat E, Beyrem H, Mahmoudi E. Neurotoxicity and oxidative stress induced by permethrin in gills of the freshwater mussel Unio ravoisieri. Chem. Ecol. [Internet]. 2017; 33(1):88–101. doi: https://doi.org/gwfbpw
Yonar ME, Mişe–Yonar SM. Changes in selected immunological parameters and antioxidant status of rainbow trout exposed to malachite green (Oncorhynchus mykiss, Walbaum, 1792). Pestic. Biochem. Physiol. [Internet]. 2010; 97(1):19–23. doi: https://doi.org/dkz8kb
Cacciatore LC, Nemirovsky SI, Guerrero NRV, Cochón AC. Azinphos–methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus. Aquat. Toxicol. [Internet]. 2015; 167:12–19. doi: https://doi.org/f7vgz7
Gagné F. Biochemical ecotoxicology: principles and methods. London (UK): Academic Press; 2014. 282 p.
Mişe–Yonar S, Yonar ME, Pala A, Sağlam N, Sakin, F. Effect of trichlorfon on some haematological and biochemical changes in Cyprinus carpio: The ameliorative effect of lycopene. Aquac. Rep. [Internet]. 2020; 16:100246. doi: https://doi.org/gg4jp6
Janaki–Devi V, NagaraniN, YokeshBabu M, Kumaraguru AK, Ramakritinan CM. A study of proteotoxicity and genotoxicity induced by the pesticide and fungicide on marine invertebrate (Donax faba). Chemosphere [Internet]. 2013; 90(3):1158–1166. doi: https://doi.org/f4mmkf
Georgieva E, Yancheva V, Stoyanova S, Velcheva I, Iliev I, Vasileva T, Bivolarski V, Petkova E, László B, Nyeste K, Antal L. Which is more toxic? Evaluation of the short–term toxic effects of chlorpyrifos and cypermethrin on selected biomarkers in common carp (Cyprinus carpio, Linnaeus 1758). Toxics [Internet] 2021; 9(6):125. doi: https://doi.org/gphn3d
