Antioxidant and anti–inflammatory effects of Helichrysum plicatum DC. subsp. plicatum extract in an experimental model of acute urolithiasis
Abstract
This study aimed to view the antioxidant and anti–inflammatory properties of Helichrysum plicatum DC. subsp. plicatum (HP) methanol extract on the urinary tract using an experimentally induced urolithiasis (U) model. The study included four groups: Group 1 was given a standard diet, Group 2 was given a diet added with HP, Group 3 was given a standard diet with induced urolithiasis, and Group 4 received an HP–supplemented diet with induced urolithiasis. Rats in Groups 2 and 4 were administered 500 mg·kg-1·day-1 of HP via gavage feeding for 21 days. Urolithiasis was induced in Groups 3 and 4 by administering 1% ethylene glycol and 1% ammonium chloride in their swig water for 21 days to create a calcium oxalate (CaOx) urolithiasis model. The study analyzed plasma concentrations of thiobarbituric acid reactive substances (TBARS), an indicator of serum oxidative stress (OS), HP, and HP levels. Additionally, oxalate (Ox), urea, calcium, and creatinine clearance levels were measured in both blood and urine, and routine histological evaluations were conducted. The results indicated important higher concentrations of HP in the groups given HP (P<0.001), while plasma TBARS concentrations were lower in Group 4 compared to Group 3 (P=0.001). The findings suggest that HP reduces OS by lowering plasma TBARS levels induced by CaOx, due to its antioxidant and anti–inflammatory properties. Furthermore, the measured biochemical measurements supported the anti–urolithiasis effects of HP. In results, this study supports the hypothesis that HP’s antioxidative and anti–inflammatory properties help prevent OS, which is a factor in stone formation, thereby preventing acute renal damage and stone formation.
Downloads
References
Ilhan M, Ergene B, Süntar I, Özbilgin S, Saltan-Çitoğlu G, Demirel MA, Keleş H, Altun L, Küpeli-Akkol E. Preclinical evaluation of antiurolithiatic activity of Viburnum opulus L. on sodium oxalate-induced urolithiasis rat model. Evid. Based Compl. Alternat. Med. [Internet]. 2014; 2014:578103. doi: https://doi.org/f6dsz4
Araújo Viel T, Diogo Domingos C, da Silva Monteiro AP, Riggio Lima-Landman MT, Lapa AJ, Souccar C. Evaluation of the antiurolithiatic activity of the extract of Costus spiralis Roscoe in rats. J. Ethnopharmacol. [Internet]. 1994; 66(2):193–198. doi: https://doi.org/d8zr4v
Atmani F, Farell G, Lieske JC. Extract from Herniaria hirsute coats calcium oxalate monohydrate crystals and blocks their adhesion to renal epithelial cells. J. Urol. [Internet]. 2004; 172(4):1510–1514. doi: https://doi.org/c4hzm3
Ramello A, Vitale C, Marangella M. Epidemiology of nephrolithiasis. J. Nephrol. 2000; 13(Suppl. 3):s45–s50. PMID:11132032
Sohgaura A, Bigoniya P. A Review on epidemiology and etiology of renal stone. Am. J. Drug Discov. Dev. [Internet]. 2017; 7(2):54-62. doi: https://doi.org/g8z65g
Khan SR. Hyperoxaluria-induced oxidative stress and antioxidants for renal protection. Urol. Res. [Internet]. 2005; 33:349–357. doi: https://doi.org/bpzzn3
Rodgers AL. Physicochemical mechanisms of stone formation. Urolithiasis [Internet]. 2017; 45:27–32. doi: https://doi.org/g8z65h
Hackett RL, Shevock PN, Khan SR. Madin-Darby canine kidney cells are injured by exposure to oxalate and to calcium oxalate crystals. Urol. Res. [Internet]. 1994; 22:197–203. doi: https://doi.org/cbzm6d
Khan SR. Role of renal epithelial cells in the initiation of calcium oxalate stones. Nephron Exp. Nephrol. [Internet]. 2004; 98(2):e55–e60. doi: https://doi.org/cxq54x
Khan SR. Pathogenesis of oxalate urolithiasis: Lessons from experimental studies with rats. Am. J. Kidney Dis. [Internet]. 1991; 17(4):398–401. doi: https://doi.org/g8z65j
Khan SR. Crystal-induced inflammation of the kidneys: results from human studies, animal models, and tissue culture studies. J. Clin. Exp. Nephrol. [Internet]. 2004; 8:75–88. doi: https://doi.org/bmhhn9
Gurocak S, Kupeli B. Consumption of historical and current phytother- apeutic agents for urolithiasis: a critical review. J. Urol. [Internet]. 2006; 176(2):450–455. doi: https://doi.org/dfjnzm
Grases F, Melero G, Costa-Bauza A, Prieto R, March JG. Urolithiasis and phytotherapy. Int. Urol. Nephrol. [Internet]. 1994; 26(5):507–511. doi: https://doi.org/fr97nt
Khan SR, Kok DJ. Modulators of urinary stone formation. Front. Biosci. [Internet]. 2004; 9:1450–1482. doi: https://doi.org/bzvj7b
Yasui T, Okada A, Hamamoto S, Ando R, Taguchi K, Tozawa K, Kohri K. Pathophysiology-based treatment of urolithiasis. Int. J. Urol. [Internet]. 2017; 24(1):32–38. doi: https://doi.org/f9ndhj
Zeng X, Xi Y, Jiang W. Protective roles of flavonoids and flavonoid-rich plant extracts against urolithiasis: A review. Crit. Rev. Food Sci. Nutr. [Internet]. 2018; 59(13):2125–2135. doi: https://doi.org/g8z65k
Facino RM, Carini M, Franzoi L, Pirola O, Bosisio E. Phytochemical characterization and radical scavenger activity of flavonoids from Helichrysum italicum G. Don (Compositae). Pharm. Res. [Internet]. 1990; 22(6):709–721. doi: https://doi.org/bcd6fd
Binu TV, Vijayakumari B. Herbal plants as a remedy for urolithiasis - A review. Int. J. Pharm. Res. Dev. 2014; 6(6):35–39.
Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Soc. Behav. Sci. [Internet]. 2011; 19:756–777. doi: https://doi.org/b79m79
Aslan M, Orhan DD, Orhan N, Sezik E, Yesilada E. In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. J. Ethnopharmacol. [Internet]. 2007; 109(1):54–59. doi: https://doi.org/bjfw45
Ozbek T, Gulluce M, Adiguzel A, Ozkan H, Sahin F, Orhan F. Antimutagenic activity of the methanol extract of Helichrysum plicatum ssp plicatum. Asian J. Chem. [Internet]. 2009 [cited 12 Jul. 2024]; 21(4):2705–2710. Available in: https://goo.su/JsLi
Demir A, Mercanoglu Taban B, Aslan M, Yesilada E, Aytac SA. Antimicrobial effect of Helichrysum plicatum subsp plicatum. Pharm. Biol. [Internet]. 2009; 47(4):289–297. doi: https://doi.org/cgkwwp
Schinella GR, Tournier HA, Prieto JM, Mordujovich de Buschiazzo P, Rios JL. Antioxidant activity of anti-inflammatory plant extracts. Life Sci. [Internet]. 2002; 70(9):1023–1033. doi: https://doi.org/fjm45w
Aydin T. Secondary metabolites of Helichrysum plicatum DC. subsp. plicatum flowers as strong carbonic anhydrase, cholinesterase and α-glycosidase inhibitors. Z. Naturforsch. C. [Internet]. 2020; 75(5-6):153–159. doi: https://doi.org/g8z65m
Sahin K, Tuzcu M, Sahin N, Akdemir F, Ozercan I, Bayraktar S, Kucuk O. Inhibitory effects of combination of lycopene and genistein on 7,12- dimethyl benz(a)anthracene-induced breast cancer in rats. Nutr. Cancer. [Internet]. 2011; 63(8):1279–1286. doi: https://doi.org/bmjrkd
Coskun H, Andic F, Daglioglu YK, Doran F, Sahin K, Tunali C, Kucuk O. Lycopene in the prevention of radiation-induced esophagitis. Nutr. Cancer. [Internet]. 2017; 69(2):319–329. doi: https://doi.org/g8z65n
Wazowicz W, Nève J, Peretz A. Optimized steps in fluorometric determination of acid-thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin. Chem. [Internet]. 1993; 39(12):2522–2526. doi: https://doi.org/g8z65p
Andic F, Garipagaoglu M, Yurdakonar E, Yurdakonar E, Tuncel M, Kucuk O. Lycopene in the prevention of gastrointestinal toxicity of radiotherapy. Nutr. Cancer. [Internet]. 2009; 61(6):784-788. doi: https://doi.org/bdx9xb
Oksay T, Yunusoğlu S, Calapoğlu M, Candan IA, Onaran I, Ergün O, Özorak A. Protective impact of resveratrol in experimental rat model of hyperoxaluria. Int. Urol. Nephrol. [Internet]. 2017; 49:769–775. doi: https://doi.org/f96tmc
Bayir Y, Halıcı Z, Keles MS, Colak S, Cakır A, Kaya Y, Akcay F. Helichrysum plicatum DC. subsp. plicatum extract as a preventive agent in experimentally induced urolithiasis model. J. Ethnopharmacol. [Internet]. 2011; 138(2):408–414. doi: https://doi.org/cq2wcw
Onaran M, Orhan N, Farahvash A, Ekin HN, Kocabiyik M, Gönül II, Şen I, Aslan M. Successful treatment of sodium oxalate ınduced urolithiasis with Helichrysum flowers. J. Ethnopharmacol. [Internet]. 2016; 186:322–328. doi: https://doi.org/g8z65q
Koul H, Kennington L, Nair G, Honeyman T, Menon M, Scheid C. Oxalate induced initiation of DNA synthesis in LLC-PK1 cells, a line of renal epithelial cells. Biochem. Biophys. Res. Comm. [Internet]. 1994; 205(3):1632–1637. doi: https://doi.org/ft9s4s
Scheid C, Koul H, Hill WA, Luber-Narod J, Kennington L, Honeyman T, Jonassen J, Menon M. Oxalate toxicity in LLC-PK1 cells: role of free radicals. Kidney Int. [Internet]. 1996; 49(2):413–419. doi: https://doi.org/bnqd3p
Thamilselvan S, Hackett RL, Khan SR. Lipid peroxidation in ethylene glycol induced hyperoxaluria and calcium oxalate nephrolithiasis. J. Urol. [Internet]. 1997; 157(3):1059–1063. doi: https://doi.org/b2k2vm
Thamilselvan S, Byer KJ, Hackett RL, Khan SR. Free radical scavengers catalase and superoxide dismutase provide protection from oxalate associated injury to LLC-PK1 and MDCK cells. J. Urol. [Internet]. 2000; 164(1):224–229. doi: https://doi.org/ftzghm
Thamilselvan S, Khan SR, Menon M. Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol. Res. [Internet]. 2003; 31:3–9. doi: https://doi.org/b4n7ff
Selvam R. Calcium oxalate stone disease: role of lipid peroxidation and antioxidants. Urol. Res. [Internet]. 2002; 30:35–47.doi: https://doi.org/c9b73x
Ilbey YO, Ozbek E, Simsek A, Cekmen M, Somay A, Tasci AI. Effects of pomegranate juice on hyperoxaluria-induced oxidative stress in the rat kidneys. Ren. Fail. [Internet]. 2009; 31(6):522–531. doi: https://doi.org/bxm8cr
Huang HS, Ma MC, Chen CF, Chen J. Lipid peroxidation and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology [Internet]. 2003; 62(6):1123–1128. doi: https://doi.org/fqqkg5
Tungsanga K, Sriboonlue P, Futrakul P, Yachantha C, Tosukhowong P. Renal tubular cell damage and oxidative stress in renal stone patients and the effect of potassium citrate treatment. Uro. Res. [Internet]. 2005; 33:65–69. doi: https://doi.org/br57zv
Ceban E, Banov P, Galescu A, Botnari V. Oxidative stress and antioxidant status in patients with complicated urolithiasis. J. Med. Life. [Internet]. 2016 [cited 18 Jul. 2024]; 9(3):259–262. PMID: 27974930. Available in: https://goo.su/5XI7n
Copyright (c) 2025 Selvinaz Yakan, Kıvılcım Eren Erdoğan, Yusuf Kenan Dağlıoğlu, Tuba Aydın, Ahmet Çakır
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
