Análisis histológico del epidídimo y los testículos en la dinámica de la ontogénesis postnatal desde un mes hasta la pubertad de los corderos Ouled Djellal
Resumen
El objetivo del estudio fue analizar la ontogenia del epitelio epididimario y seminífero en el testículo de los corderos Ouled Djellal a lo largo del desarrollo postnatal hasta la pubertad. Se utilizaron un total de 24 corderos Ouled Djellal, con edades comprendidas entre 1 y 8 meses, seleccionando tres corderos en cada mes de edad. Los corderos fueron castrados quirúrgicamente mensualmente, y se realizó histología de rutina junto con histomorfometría en los tejidos epididimarios y testiculares procesados utilizando el software AxioVision Rel 4.6. El análisis estadístico reveló que el peso testicular aumentó a un ritmo acelerado entre los 4 y los 8 meses postnatales, coincidiendo con modificaciones significativas en la histología testicular. Estas modificaciones abarcaron incrementos significativos en la medida de los túbulos seminíferos y un incremento en los niveles de testosterona. Se encontró que el diámetro ductal, el diámetro luminal y la altura del epitelio eran estadísticamente diferentes (P<0.05) entre las regiones caput, corpus y cauda del epidídimo, con los niveles máximos registrados durante la pubertad. A los 1 y 2 meses de edad, había dos tipos de células presentes en el epitelio seminífero: células de soporte y gonocitos. A los 3 meses de edad, se formaron los primeros espermatogonios a partir de gonocitos, coincidiendo con el peso promedio de los testículos alcanzando 4 g. La espermatogénesis se inició cuando los gonocitos sufrieron mitosis, dando lugar a progenitores que se diferenciaron en espermatogonios. A los 4 y 5 meses de edad, se observó inicialmente un lumen y espermatocitos primarios (espermatocitos I) en los túbulos seminíferos. A los 6 meses de edad, espermatocitos secundarios y espermatides redondas formaron una única fila dentro de los túbulos seminíferos. A los 7 y 8 meses de edad, todas las generaciones de células madre germinales se encontraban en los seminiferous tubules. A los 8 meses de edad, se observaron espermatozoides en varios segmentos del epidídimo en la especie Ouled Djellal, dando inicio a la etapa de pubertad.
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Derechos de autor 2025 Yamina Belkhiri, Farida Bouzebda–Afri, Zoubir Bouzebda, Souheyla Benbia, Ramzi Lamraoui
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
