Antioxidant and anti–inflammatory effects of Helichrysum plicatum DC. subsp. plicatum extract in an experimental model of acute urolithiasis

Selvinaz Yakan; Kıvılcım Eren Erdoğan; Yusuf Kenan Dağlıoğlu; Tuba Aydın; Ahmet Çakır

doi:10.52973/rcfcv-e35502

Selvinaz Yakan Ağrı İbrahim Çeçen University, Eleşkirt Celal Oruç School of Animal Production, Animal Health Department. Ağrı, Türkiye. https://orcid.org/0000-0002-6274-9012
Kıvılcım Eren Erdoğan Çukurova University, Faculty of Medicine, Department of Pathology. Adana, Türkiye https://orcid.org/0000-0002-4951-8703
Yusuf Kenan Dağlıoğlu Kirşehir Ahi Evran University, Faculty of Medicine, Department of Microbiology. Kırşehir, Türkiye https://orcid.org/0000-0003-3140-2937
Tuba Aydın Ağrı İbrahim Çeçen University, Faculty of Pharmacy. Ağrı, Türkiye https://orcid.org/0000-0002-7653-6480
Ahmet Çakır Kilis 7 Aralık University, Faculty of Science and Literature, Department of Chemistry. Kilis, Türkiye https://orcid.org/0000-0003-1672-1438

DOI: https://doi.org/10.52973/rcfcv-e35502

Palabras clave: Cristales de oxalato de calcio, urolitiasis, rata, sustancia reactiva al ácido tiobarbitúrico (TBARS), Helichrysum plicatum DC. subsp. plicatum

Resumen

Este estudio tuvo como objetivo evaluar las propiedades antioxidantes y antiinflamatorias del extracto de metanol de Helichrysum plicatum DC. subsp. plicatum (EP) en el tracto urinario, utilizando un modelo de urolitiasis (U) inducido experimentalmente. El estudio incluyó cuatro grupos: al Grupo 1 se le administró una dieta estándar convencional, al Grupo 2 se le administró una dieta complementada con EP, al Grupo 3 se le administró una dieta estándar con urolitiasis inducida y el Grupo 4 recibió una dieta complementada con EP y con urolitiasis inducida. A las ratas de los Grupos 2 y 4 se les administró 500 mg·kg^-1·dia^-1 de EP mediante alimentación por sonda durante 21 días. La urolitiasis se indujo en los Grupos 3 y 4 mediante la administración de 1 % de etilenglicol y 1 % de cloruro de amonio en su agua potable durante 21 días para crear un modelo de urolitiasis por oxalato de calcio (CaOx). El estudio analizó las concentraciones plasmáticas de sustancias reactivas al ácido tiobarbitúrico (TBARS), un indicador de estrés oxidativo (EO), así como los niveles de EP en el suero. Además, se midieron los niveles de oxalato (Ox), urea, calcio y la depuración de creatinina en sangre y orina, y se realizaron evaluaciones histológicas de rutina. Los resultados indicaron concentraciones significativamente más altas de EP en los grupos que recibieron EP (P<0,001), mientras que las concentraciones plasmáticas de TBARS fueron más bajas en el Grupo 4 en comparación con el Grupo 3 (P=0,001). Los hallazgos sugieren que EP reduce el EO al disminuir los niveles plasmáticos de TBARS inducidos por CaOx, debido a sus propiedades antioxidantes y antiinflamatorias. Además, las mediciones bioquímicas realizadas respaldaron los efectos antiurolíticos de EP. En resumen, este estudio respalda la hipótesis de que las propiedades antioxidantes y antiinflamatorias de EP ayudan a prevenir el EO, que es un factor en la formación de cálculos, previniendo así el daño renal agudo y la formación de cálculos.

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Efectos antioxidantes y antiinflamatorios del extracto de Helichrysum plicatum DC. subsp. plicatum, en un modelo experimental de urolitiasis aguda

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