Identification of acidic pigments in solutions of crude rhamnolipids produced by a strain of Pseudomonas aeruginosa
Abstract
Recent studies indicate that the unpurified rhamnolipids produced by P. aeruginosa are more efficient in removing oil, due to the presence of other metabolites, primarily acidic. Among the main acid metabolites are organic acids and some phenazines pigments. In order to identify acidic pigments in solutions of crudes rhamnolipids produced by P. aeruginosa strain BioMI-E5, in this study were purified them by adsorption chromatography to hydrophobic and amphiphilic substances with Amberlilta XAD 2. They were separated by a thin layer chromatography using silica gel plates and then were analyzed by Infrared Spectroscopy Fourier Transform (FTIR). Four fluorescent pigments were detected in solutions of crude rhamnolipids. FTIR analysis showed functional groups which correspond to those present in phaenazines pigments most frequently registered in cultures of P. aeruginosa . The presence of carboxylic groups was detected which demonstrates the acid character in these pigments. The results confirm that these pigments are part of the set of acidic metabolites thatare present in crudes solutions of rhamnolipids pruduced by P. aeruginosa strain BioMI-E5.
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