Revista Electrónica:
Depósito Legal: ppi 201502ZU4665 / / ISSN electrónico: 2477-944X
Revista Impresa:
Depósito Legal: pp 199102ZU46 / ISSN 0798-2259
MARACAIBO, ESTADO ZULIA, VENEZUELA
Vol. XXX (3) 2020
UNIVERSIDAD DEL ZULIA
REVISTA CIENTÍFICA
FACULTAD DE CIENCIAS VETERINARIAS
DIVISIÓN DE INVESTIGACIÓN
117
Revista Cientíca, FVC-LUZ / Vol. XXX, N° 3, 117 - 125, 2020
PRODUCTIVE PERFORMANCE OF THE “GREEN TERROR”
(Andinoacara rivulatus) FISH DURING THE FATTENING STAGE WHEN
FED DIETS WITH PASSION FRUIT CAKE (Passiora edulis)
RENDIMIENTO PRODUCTIVO DE LA ESPECIE DEL PEZ NATIVO “TERROR VERDE” (Andinoacara rivulatus)” DURANTE LA
ETAPA DE ENGORDE CUANDO SE ALIMENTA CON DIETAS CON TORTA DE MARACUYÁ (Passiora edulis)
Edison Mazón-Paredes
1*
, José Luis Guzmán-Guerrero
2
, Marlene Mazón-Paredes
3
,
Antón García-Martinez
4
, Carlos Mazón-Paredes
5
and Marcelino Herrera-Rodriguez
6
1
Facultad de Ciencias Pecuarias, Universidad Técnica Estatal de Quevedo, Km 6 ½ vía a El Empalme cruce a Mocache, 120501,
Quevedo, Los Ríos, Ecuador: emazon@uteq.edu.ec
2
Departamento de Ciencias Agroforestales, Escuela Técnica Superior de
Ingeniería, Universidad de Huelva, “Campus de Excelencia Internacional Agroalimentario, ceiA3”, Campus de la Rábida, 21819 Palos
de la Frontera, Huelva, España; guzman@uhu.es
3
Facultad de Ciencias de la Vida, Universidad Estatal Amazónica, Calle Teniente
Hugo Ortíz E45, 160150, Puyo, Pastaza, Ecuador: mmazon@uea.edu.ec
4
Facultad de Veterinaria, Universidad de Córdoba, Campus
de Rabanales, s/n 14071, Córdoba, España; pa1gamaa@uco.es
5
Facultad de Ciencias Pecuarias, Universidad Técnica Estatal de
Quevedo, Km 6 ½ vía a El Empalme cruce a Mocache, 120501, Quevedo, Los Ríos, Ecuador: cmazon@uteq.edu.ec
6
Instituto de Investigación y Formación Agraria y Pesquera (IFAPA), Centro Agua del Pino. Km 4, Carretera El Rompido-Punta Umbría,
21450 Cartaya, Huelva, España; marcelino.herrera@dcaf.uhu.es
*Corresponding author: email: emazon@uteq.edu.ec, Telf.: +593 52783948, Cell.: +593 983268968
ABSTRACT
The objective of the present work was to evaluate the eect of
diets containing passion fruit cake on the productive performance
of the “green terror” (Andinoacara rivulatus)”. Fish were fed four
passion fruit diets (T1: 0%, T2: 3%, T3: 6%, T4: 9%) for 30 days.
The weight increase (WI), relative weight increase (RWI), growth
rate (GR), incremental growth rate (IGR), feed conversion ratio
(FCR), dry matter digestibility, gross protein digestibility (GPD),
gross energy digestibility (GED), protein eciency rate (PER),
productive value of protein (PVP), and feed cost were assessed.
There were no dierences in the nal weight, WI, RWI, GR and
IGR values between T1 and T2; only the diet in T3 and T4 showed
poorer results than the T1 diet. FCR was higher in the control diet
(T1) and in T4 than in T2 and T3. As the percentage of passion
fruit increased, the cost of the diet decreased. GPD and GED did
not exhibit dierences in T1, T2 and T3, only DT T4 had lower
digestibility values. PER of control DT T1 had a better coecient
than T2, T3 and T4. Signicant dierences were found in PVP
between the control DT, T2 and T4. However, the lowest values
were obtained with this latter DT. The addition of up to 3-6%
passion fruit did not aect the yield, and the cost of the diet was
signicantly reduced.
Key words: Andinoacara rivulatus; apparent digestibility;
fattening stage; experimental diets; P a s s i o r a
edulis
RESUMEN
El objetivo fue evaluar el efecto de las dietas, que contenían
maracuyá, en el rendimiento productivo del “terror verde”. Los
peces fueron alimentados con cuatro dietas de torta de maracuyá
(T1: 0%, T2: 3%, T3: 6%, T4: 9%) durante 30 días. El aumento de
peso (WI), aumento de peso relativo (RWI), tasa de crecimiento
(GR), tasa de crecimiento incremental (IGR), conversión de
alimento (FCR), digestibilidad de materia seca, de la proteína
bruta (GPD) y de la energía bruta (GED), tasa de eciencia
proteica (PER), valor productivo de la proteína (PPV) y el costo
de alimentación fueron evaluados. No hubo diferencias en los
valores de peso nal, FW, WI, RWI, GR e IGR entre T1 y T2; solo
la dieta en T3 y T4 mostró resultados peores que la dieta T1. El
FCR fue mayor en la dieta control (T1) y en T4 que en T2 y T3.
A medida que el porcentaje de racuyá aumentaba, el costo de la
dieta disminuía. GP y GE no mostraron diferencias en T1, T2 y
T3, sólo el ensayo T4 tuvo valores de digestibilidad más bajos. El
PER del ensayo de control tenía un coeciente mejor que T2, T3
y T4. Se encontraron diferencias signicativas en el PVP entre el
ensayo de control, T2 y T4. Sin embargo, los valores más bajos
se obtuvieron con este último ensayo. La adición de hasta 3-6%
de maracuyá no afectó el rendimiento, y el costo de la dieta se
redujo signicativamente.
Palabras clave: Andinoacara rivulatus; digestibilidad aparente;
fase de engorde;dietas experimentales;
Passiora edulis
Recibido: 01/07/2020 Aceptado: 26/08/2020
118
Productive performance of the “green terror / Mazón, E. y col.
INTRODUCTION
Currently in Ecuador, there is no constant and adequate supply
of plant resources for high protein certied organic preparation of
diets for sh farming, highlighting the need to explore alternative
plant resources (PR) to replace conventional protein sources,
such as passion fruit cake (PFC) [18, 22, 31].
Organic aquaculture diers from conventional aquaculture in
that it is focused on production in harmony with the environment,
employing practices that seek to duplicate the natural conditions
of organisms [8], striving always be committed to social, economic
and sustainability factors, including the rational use of resources
for feed [31].
The high cost of traditional energy products used in animal feed
has sparked the search for new products and an evaluation of
their nutritional potential. One of these crops is PFC, which is not
well known, though it has great potential in feeding animals due
to its very low cost.
PF is a source of protein, minerals, carbohydrates and fats. PF
has an energy value of 78 calories, 2.4 grams (g) of carbohydrates,
5 g of calcium, 17 miligram (mg) of phosphorus, 0.3 mg of iron,
684 mg of activated vitamin A, 0.1 mg of vitamin B2 (riboavin),
2.24 mg of niacin and 20 mg of vitamin C. In Ecuador there are
around 28 thousand hectares (hes) planted PF with an average
yield of about 14 tons (T) per hes. The main variety is Passiora
edulis avicarpa (yellow fruit), as its production per hes is higher
and it is ideal for processing. It is estimated that a well-managed
plantation can yield 8-10 T per hes in the rst year (yr), 15-20 T in
the second yr and 12-14 T in the third yr [2].
Duchi and Pazmiño [10] have showed that the industrialization
of PF produces by-products such as PF peel. Originally, these
industrial by-products were solid waste that contaminated the
environment (soil, air and water); however, advances in alternative
animal production methods in the tropics have permitted fresh
and dry peel to be used as a supplement in dairy (Bos-taurus) and
beef cattle diets Bos indicus.
Mazón [21] has reported that PFC has a high content of dry
matter (DM), a general average of 92.85% and an average value
of 67.32% organic matter (OM). The gross protein (GP) and
ether extract (EE) showed high values of 23.95 and 11.81%,
respectively. Gross ber (GF) and nitrogen-free extract (NFE) had
an average value of 46.27 and 20.98%. The average values of
neutral detergent ber (NDF) and acid detergent ber (ADF) were
therefore high, at 72.47 and 69.29%. The average ash content
was 2.07%, and the general average for calcium and phosphorus
was 2.85 and 0.32%. The average value of gross energy (GE)
was 5.19 mega calories per kilogram of dry material (Mcal·kg
-1
DM).
The development of high nutritional quality, low environmental
impact and economically protable diets for sh farmers is a
pressing need in the sh feed industry, especially for intensive
production systems. Rations having these characteristics are
possible when formulated with ingredients of high nutritional
value, based on the ingredient digestibility data of each particular
species [17].
The rapid expansion of sh farming in recent yr, like other forms
of intensive animal production, requires improved nutrition as well
as complete rations [13]. Feed is the most signicant production
cost; the increased complexity of the feed required in aquaculture
means that this item normally exceeds 70% of the total cost, and
therefore justies the eorts to understand the principles of sh
nutrition and feeding [1, 9].
Green terror (Andinoacara rivulatus), the target species of
this work, is native to Peru and Ecuador. Males can reach 30
centimeter (cm), while females do not exceed 20 cm. They
prefer rather high temperatures, not below 25 °C. They are
very adaptable to pH and water hardness conditions, but they
do not tolerate the presence of nitrogenated compounds in the
water (such as ammonium); it is thus essential to change the
water continuously and have a good ltration system when they
are grown in captivity. It is an omnivorous sh, so it accepts any
type of food. However, due to their voracity, they should not be
overfed, as they will always seem dissatised. They are territorial
and aggressive, so they should be housed in large ponds and
never share the same place with smaller sh [22].
Passion fruit cake (PFC) has been used for sh, poultry and
ruminants feeding programs as a source of protein and energy
in monogastric and ruminant diets. In the case of monogastrics,
it is used to replace shmeal as the source of animal protein and
corn (Zea mays) as the energy source, as both products are more
expensive. There are reports of research into the use of PFC in
the diets of native sh such as Andinoacara rivulatus to replace
industrial shmeal as the source of protein, and yellow corn as the
source of energy.
Therefore, with the aim of expanding the knowledge of PFC
and its uses in animal feed, this study evaluated the eect of
diets based on PFC on the yield of the native “green terror” in the
fattening stage during the dry season.
MATERIAL AND METHODS
Fish housing, feeding and sampling
Four hundred sh with an initial weight of 44.2 ± 1.5 g were
used, and the stocking density was 25 sh per cage (1.11kg/
meter (m
3
)). There were 16 experimental cages 1 m long x 1 m
wide x 1 m high, covered with 6 x 8 cm extruded plastic mesh,
and the tank was 30 x 20 m and 0.8 m deep. An electric balance
of 5 kg capacity and a minimum weight of 1 g was used for the
119
Revista Cientíca, FVC-LUZ / Vol. XXX, N° 3, 117 - 125, 2020
biometric data of the sh. A 30 x 1.5 m platform was used to
deliver the experimental diets. The pond did not have aeration
equipment because it was supplied with a constant 15 liter (L)/
minutes (min) ow of highly oxygenated, double ltered water
from a 0.25 hes reservoir. The water temperature was 20 ± 0.5
°C. The dissolved oxygen content of the water in the tanks was
maintained at 11 ± 1 mg/L and the pH was 7.5 ± 0.5. Nitrogenated
wastes (unconsumed food and excreted organic matter) were
removed daily using a Monge drainage system.
The sh had seven days (d) o adapt to the experimental cages
before the start of the experiment and were fed high-protein
extruded feed to accustom them to consuming concentrated feed.
Four experimental diets were prepared with dierent percentages
of PFC (0, 3, 6 and 9%) formulated by the trial and error method
to represent isoproteic (35% total protein) and isocaloric (3000,
3006, 3006 and 3013 kilocalories (Kcal)/kg) diets, using the
inputs and quantities shown in TABLE I. The experimental diet
was oered ad libitum four times a d (0800, 1100, 1300 and
1500 hours (h) for 30 d. As a prophylactic measure, the sh were
treated with a solution of methylene blue (5g/10 L every 30 d)
to prevent Ichthyopthirius fungus and pathogenic bacteria. After
each sampling, a solution of methylene blue diluted in water was
used to prevent contamination by fungi and bacteria.
Dietary Treatments (DT)
The experimental DT were: T1 (0%), T2 (3%), T3 (6%) and
T4 (9%), depending on the percentage of PFC in the diet.
Experimental diets underwent a pelleting process involving
agglomeration through the addition of binders such as bentonite
and molasses, and water. Then they were passed through a
ne diameter matrix to form spherical particles (pellets), which
were hardened by steam cooking in a rotary kiln. A completely
randomized design with four DT were used (4 diets with dierent
percentages of PFC, four repetitions (4 cages) per DT, and
the data were collected for 30 d. The manual on Nile Tilapia
(Oreochromis niloticus) nutritional needs by Torres-Novoa [30],
University of The Llanos, Colombia, was used as a reference,
based on the inputs and the amounts indicated in TABLE I.
The experimental DT (diets) and their proximal composition of
experimental diets are shown in TABLE II.
Determination of biological parameters
The ingredients, diets and feces were analyzed to determine
their proximal composition according to the methodologies
of Ocial Methods of Analysis [25], dry matter (DM) by kiln
TABLE I
COMPOSITION OF EXPERIMENTAL DIETS CONTAINING PASSION FRUIT
CAKE USED IN THE FATTENING PHASE OF Andinoacara rivulatus
Ingredients (%)
1
Experimental diets (%)
T1 T2 T3 T4
Corn
Wheat bran
Rice our
Soybean cake
Fishmeal
Soybean oil
Passion fruit cake
Salt
Antifungal
2
Methionine
Antioxidant
3
Choline Chloride
Bentonite
Pre-mix
4
Enzyme
5
12.90
0.00
11.50
32.00
36.00
3.80
0.00
0.10
0.05
0.20
0.05
0.10
3.00
0.10
0.10
5.80
2.00
16.00
37.60
30.00
1.80
3.00
0.10
0.05
0.20
0.05
0.10
3.00
0.10
0.10
3.40
3.00
17.30
38.00
28.00
0.50
6.00
0.10
0.05
0.20
0.05
0.10
3.00
0.10
0.10
2.70
0.00
8.10
43.50
27.00
6.00
9.00
0.10
0.05
0.20
0.05
0.10
3.00
0.10
0.10
1
1
Air dried food; T1: 0% Passion Fruit Cake, T2: 3% Passion Fruit Cake, T3: 6% Passion Fruit Cake, T4: 9% Passion Fruit Cake
2
Mollejosanitin
3 Endox
4
Rovimix Pre-mix: Vitamin A, D3, K, E, B1, B2, B6, Nicotinic Acid, Calcium Pantothenate, Biotin, Folic Acid, Choline, Inositol and Vitamin C
5
Avizyme 1502 (600 U g
-1
endo-1,4 beta xylanase EC 3,2,1,8; 8000 U g
-1
subtilisin - protease- EC 3,4,21,62; 800 U g
-1
alpha amylase EC
3,2,1,1.).
120
Productive performance of the “green terror / Mazón, E. y col.
drying at 105°C/24 h, gross protein (% N x 6.25) by the Kjeldahl
method, lipids by solvent extraction with soxlhet apparatus,
ash by incineration at 550°C/6 h and gross energy using a Parr
Instruments 121AE adiabatic calorimetric pump (USA).
The experiment was carried out in a fattening pond where the
16 experimental cages containing 25 sh each were located, to
which the PFC diets were fed to establish the productive yield of
the native sh. Once the fattening period was over, the sh were
transferred to metabolic tanks and introduced into each of them
for processing.
To determine the digestibility of the diets, the total stool
collection method was used in each of the DT and repetitions
based on the modied Guelph system [28]. A cylindrical tank with
a conical bottom containing 200 L of water was used; oxygen was
provided by means of a portable oxygenator located at the top of
the tank, and at the bottom, a 20 cm long tube of two inches in
diameter length with a ball valve in the middle and a screw cap
on the end was inserted, which allowed the feces to be collected
every 6 h over a total period of 24 h [16]. The feces obtained in
each collection were placed in a 50: cubic centimeter container,
the excess moisture was removed and they were stored in a
refrigerator (Indurama, Ecuador) at 4 °C until the corresponding
bromatological chemical analysis was performed.
The yield of Andinoacara rivulatus was evaluated by measuring
following zootechnical parameters.
Feed conversion ratio (FCR):
Weight increase (WI) is the weight increase per unit of time due
to food consumption (energy and protein) at a certain temperature.
Where:
Wf = nal weight
Wi = initial weight
The relative weight increase (RWI) is the increase in weight per
unit of time at dierent ages due to food consumption (energy and
protein) within a given temperature range.
TABLE II
PROXIMAL COMPOSITION OF EXPERIMENTAL DIETS WITH PASSION FRUIT
CAKE USED FOR THE JUVENILE PHASE OF Andinoacara rivulatus
Proximal composition (%) T1 T2 T3 T4
Request
1
Digestible dietary
energy (Kcal kg-
1
)
Total protein
Fiber
Calcium
Phosphorus
2
Arginine
Lysine
Met + Cys
Tryptophan
3000
35.00
3.10
1.84
1.50
1.91
2.09
1.11
0.36
3002
35.00
4.20
1.58
1.40
1.96
2.03
1.09
0.36
3003
35.00
4.90
1.49
1.40
1.98
1.98
1.08
0.36
3014
35.00
6.30
1.47
1.30
2.36
2.36
1.12
0.39
3000
35.00
---
1.00
0.80
0.94
0.94
0.35
0.30
1
Total phosphorus in the diet
121
Revista Cientíca, FVC-LUZ / Vol. XXX, N° 3, 117 - 125, 2020
Where:
Wf = nal weight
Wi = initial weight
The growth rate (GR) is a measure of the average increase or
decrease in weight over a period of 30 d due to the amount of
feed consumed and the water temperature.
Where:
Wf = nal weight
Wi = initial weight
t = time
The incremental growth rate (IGR) is a measure of the average
weight gain over a period of 30 d due to the amount of feed
consumed and the water temperature.
Where:
Wf = nal weight
Wi = initial weight
t = time
Net feed consumption (NFC) is the amount of complementary
feed consumed weekly minus the amount of residual feed of the
measure weighed weekly:
NFC = Weight of feed consumed (g) - Weight of waste (g)
The protein eciency rate (PER) is the weight gained by an
animal for each unit of protein in the feed and was calculated for
each DT using the following equation [3]:
The protein production value (PVP) were calculated for each
assay using the following equation:
Apparent digestibility coecient
The apparent digestibility coecient (DC) was calculated by
collecting all the feces in each of the DT and repetitions using the
modied Guelph system [28]. This method employs a cylindrical
tank with a conical bottom (metabolic aquarium) where water
and oxygen are fed continuously from the top and there is a
feces collection tube at the bottom [1, 4, 14, 15]. Subsequently,
a bromatological analysis was performed on the feces extracted
and samples of the experimental diets used in the studies.
The digestibility of the experimental sh diets was determined
by the direct method, also called the total collection method. It
consisted of the quantitative collection and analysis of all feces
produced. The digestibility coecient (DC) was calculated as
follows:
Statistical analysis
A completely randomized design was used with four DT and
four repetitions. All zootechnical and biological parameters were
analyzed with an ANOVA analysis of repeated measurements
using the General Linear Model (GLM) of the Statistical Analysis
System Software statistical package (Workow Studio 1.3)
System for Windows 11, Copyright 2016 by SAS Institute Inc.,
Cary, NC, USA). The model considered the percentage of PFC
(0, 3, 6 and 9%), analyzed as repeated measurements on the
same experimental units. When signicant dierences were
detected between the means of factors with more than two levels,
they were subjected to a multiple comparison of means using
Honestly-signicant-dierence (HSD) of Tukey values expressed
as mean + standard error of mean (SEM).
The unrestricted, randomized experiment consisted of four DT,
four repetitions and 25 sh per experimental cage:
The mathematical model is shown below:
Yij = μ + Ti + εij
Where:
Yij = Observations for dependent variables.
μ = Average population
Ti = “i-th” eect of the DT
εij = Random eect (Experimental error).
The Tukey test was used for the comparison of means, with a
probability of 5%.
RESULTS AND DISCUSSION
Digestibility of experimental diets
By feeding experimental diets based on PFC, it was possible to
establish the digestibility coecients of DM, GP and GE for this
tropical native species during the fattening period, TABLE III.
When comparing the control diet T1 with diets T2, T3 and T4,
signicant dierences were found in the apparent digestibility
coecients of the dry material (P<0.05), but no signicant
122
Productive performance of the “green terror / Mazón, E. y col.
dierences (P>0.05) were found between DT T1, T2 and T3 and
DT T4 in the digestibility of GP and GE. Moreover, this latter
DT, which had the highest percentage (9% PFC), had the lowest
digestibility coecient compared to the other DT (P≤0.05).
The results obtained in this study agree with Vásquez et al. [31]
in a study of tilapia (Oreochromis spp) and Vasquez et al. [32]
for cachama (Piaractus brachypomus). The apparent digestibility
coecients achieved in this study are generally consistent with
those described in other studies involving tilapia (Oreochromis
niloticus) and cachama with similar DM, GP and GE. There
are discrepancies among the results published in the literature,
and according to a consensus among researchers, this may be
caused by dierences in the methodologies used to determine
the coecients or process the diets, dierences in the amounts
added of the ingredient being studied, the type of control diet
used Anderson et al. [6], Boscolo et al. [8], Furuya et al. [13],
Guimarães et al. [15], Masagounder et al. [20] the method of fecal
collection Meurer et al. [23], the size of the sh, the equation used
to calculate the coecients Foster [12] and the process used to
prepare the experimental diets [5]. The selection of the most
digestible ingredients makes it possible to improve zootechnical
indices and reduce water pollution [26].
Other authors state that the low digestibility of vegetable by-
products such as passion fruit cake PFC is normally attributed
to high levels of non-starch polysaccharides (NSP) Wing [34]
or cell wall composition Dusterhoft and Voragen [11]. Wing [34]
states that these antinutritional factors reduce the digestibility
and uptake of the nutrients in PFC, either by direct encapsulation
of the nutrients or by increasing the viscosity of the intestinal
content, thus reducing the rate of hydrolysis and absorption of the
nutrients in the feed. This could explain the poorer yields found
with the 9% diet compared to the control diets. The addition of
proteolytic, brolytic or carbohydrate degrading enzymes to PFC
diets has great potential for releasing unavailable nutrients and
energy.
Signicant dierences (P≤0.05) were found in PER between
all DT and signicant dierences (P≤0.05) were found in PVP
between the control DT T1, T2 and T4. However, the lowest
values were obtained with this later DT (9% PFC).
Regarding the yield of green terror in the fattening stage, the
results obtained for the PER and PVP in this study are relatively
low compared to the results obtained by Bermúdez et al. [7] with
Nile tilapia, Yudy et al. [35] with yamú (Brycon siebenthalae),
Madrid [19] with gulf corvina (Cynoscion othonopterus) juveniles,
and Madrid [19] with sabaleta (Briycon henni).
This could be because this native sh has dierent feeding
habits from those of the sh species compared, and the partial
replacement of the shmeal could have reduced the eciency
rates PVP and PER. Moreover, it should be noted that this native
sh has not undergone genetic improvement in any physiological
stage, and it has not been exploited using intensive or semi-
intensive production systems based on balanced feed, that would
have allowed its organism to adapt to consuming balanced feed.
When comparing the mortality between trails, it can be seen that
it was very low, and sh deaths occurred due to handling during
initial and nal weighing, measurement of external anatomical
dimensions and sh transfer to the metabolic aquariums of
the experiment. Mortality can also be attributed to attacks by
Ichthyopthirius fungi and external parasites. The mortality was
therefore not due to the experimental diets (TABLE IV).
Productive performance
The yield of the native species “green terror” fed dierent
isoproteic and isocaloric diets is shown in TABLE IV. After
TABLE III
APPARENT DIGESTIBILITY COEFFICIENTS (DMD, GPD AND GED) FOR
Andinoacara rivulatus JUVENILES FED DIETS CONTAINING PASSION FRUIT CAKE
Variable
1
Dietary Treatments (DT)
T1 T2 T3 T4
Dry matter digestibility (%). 58.65 + 0.35 a 58.20 + 0.35 b 57.35 + 0.35 c 50.58 + 0.35 d
Gross protein digestibility
(%).
84.48 + 0.75 a 84.53 + 0.75 a 84.42 + 0.75 a 79.58 + 0.75 b
Gross energy digestibility
(%).
77.58 + 0.69 a 77.10 + 0.69 a 77.45 + 0.69 a 71.70 + 0.69 b
Protein eciency rate (%) 0.33 + 0.02 a 0.31 + 0.02 b 0.26 + 0.02 c 0.20 + 0.02 d
Productive value of protein
(%).
0.60 + 0.004 a 0.59 + 0.004 b 0.59 + 0.004 ab 0.56 + 0.004 c
abc
Averages with dierent letters in the same line dier statistically according to the Tukey test
1
These are the means (± EEM) of 25 sh housed in a cage (Experimental Unit) with four replicates per trial. T1: 0% Passion Fruit Cake,
T2: 3% Passion Fruit Cake, T3: 6% passion fruit cake and T4: 9% passion fruit cake.
123
Revista Cientíca, FVC-LUZ / Vol. XXX, N° 3, 117 - 125, 2020
supplying the experimental diets during a research period of 30 d
during the fattening season, no signicant dierences were found
(P<0.05) for Wf, WI, RWI, GR and IGR of “green terror”, between
DT T1 and T2 when PFC was added; however, these two DT
did show dierences compared to DT T3 and T4, which showed
poorer results than DT T1.
Some initial studies suggest that palm kernel cake can be
tolerated even up to 30% in rations for catsh (Clarias gariepinus)
and 20% for tilapia, well above the levels in this work, without any
adverse eects on growth or yield [27].
With the same species, Wan [33] and Wing [34] found
satisfactory results up to a level of 20%. The yield did not vary
when cachama were fed this alternative source of protein Vasquez
[31], so these can also serve as a reference for the use of PFC.
The results do not of this work agree with those obtained with
palm kernel cake (PKC) for diets of red tilapia ngerlings, which
also incorporated PKC up to a level of 8% without aecting the
sh yield, which suggests that higher levels could be used. Other
authors have also used PKC as an organic ingredient for the
partial or total replacement of shmeal for aquatic species up to
8% without negative eects on the yield [32].
Net feed consumption exhibited signicant dierences (P≤0.05)
between DT T1, T2, T3 and T4. The consumption of feed
decreased when the PFC in the diets of the sh was increased.
Signicant dierences in feed conversion ratio were found
(P<0.05) between DT T1 and T4, in compared to DT T2 and T3,
and the best conversions occurred in these DT compared to the
control DT.
As with many ingredients of vegetable-based and oil-seed
feeds, there are several factors that can limit the inclusion of
PFC in sh diets. These include its relatively low protein content,
possible amino acid deciency, and antinutritional factors [33].
However, in the present study, diets with various percentages
o PFC used 59% total protein export quality shmeal, which
provided adequate levels of amino acids. Moreover, it was
supplemented with nutritional additives including a pre-mix of
vitamins and minerals and synthetic methionine.
Therefore, amino acid deciency does not seem to be the cause
of the poorer yields obtained with the diet containing the highest
percentage (9%), despite the high percentage of crude ber that
could possibly have had a lower availability of essential amino
acids. This native species has not been exploited in captivity nor
fed balanced feed, so its digestive system has not adapted to
this type of food and did not produce the necessary enzymes for
digesting these nutrients (protein, oil, ber, etc.).
Regarding feed costs, the price per kg of the diet prepared with
the highest shmeal content (0% PFC) was seen to be higher,
as its cost was quite high. When the shmeal in the diet was
partially replaced, the cost per kilogram of feed (3, 6 and 9% PFC)
was reduced. It should be noted that the addition of amino acid
supplements was not necessary in diets containing PFC, so there
was no increase in the added cost, which is why it is considered
to be a cheaper alternative to shmeal.
Ng et al. [24] in work carried out with tilapia that were fed PFC
pre-treated with enzymes for commercial feed, consistently found
greater eciency in growth and feed utilization compared to sh
fed similar levels of raw PKC. They then indicated that up to
30% PKC treated with enzymes could be incorporated into the
TABLE IV
EFFECT OF DIETS CONTAINING DIFFERENT PERCENTAGES OF PASSION FRUIT CAKE ON THE PRODUCTIVE
PERFORMANCE INDEX OF Andinoacara rivulatus IN THE FATTENING PHASE
Variable
1
Dietary Treatments (DT)
T1 T2 T3 T4
Final weight (g) 49.25+ 1.10 a 49.00+1.10 a 47.25+1.10 b 45.00+ 1.10 c
Weight increase (g) 14.25+ 1.10 a 14.00+ 1.10 a 12.25+ 1.10 b 10.00+ 1.10 c
Relative weight increase (g). 40.70+3.16 a 40.00+3.16 a 35.00+ 3.16 b 28.58+ 3.160c
Growth rate (%) 47.50+ 3.68 a 46.68+3.68 a 40.82+ 3.68 b 33.35+ 3.68 c
Incremental growth rate (%) 1.15+ 0.08 a 1.12+ 0.08 a 1.00+ 0.08 b 0.85+ 0.086 c
Net feed consumption (g) 80.00+ 0.80 a 78.00+0.80 b 76.00+ 0.80 c 74.00+0.80 d
Feed conversion ratio (g) 1.63+ 0.02 a 1.59+ 0.02 b 1.61+ 0.02 b 1.64+ 0.02 a
Feed cost ($ Kg.
-1
) 0.72+ 0.001 a 0.71+ 0.001 b 0.70+ 0.001 c 0.69+ 0.001 d
Mortality (%) 5 4 2 3
abc
Averages with dierent letters in the same line dier statistically according to the Tukey test
1
These are the means (± EEM) of 25 sh housed in a cage (Experimental Unit) with four replicates per trial. T1: 0% Passion Fruit Cake,
T2: 3% Passion Fruit Cake, T3: 6% passion fruit cake and T4: 9% passion fruit cake.
124
Productive performance of the “green terror / Mazón, E. y col.
diet of red tilapia without slowing its growth signicantly, which
was corroborated by Wing [34]. Therefore, the enzyme addition
is key to achieve higher PFC content without negative eects on
zootechnnical parameters and performance. That is the reason
why an enzymatic complex based on protease, xylanase and
amylase was added in our experimental diets.
CONCLUSIONS
Green terror has high capacity to utilize the nutrients in diets
prepared with up to 3% PFC, supplemented by an enzymatic
complex during fattening, without aecting the yield of sh in terms
of nal weight, weight increase, relative weight increase, growth
rate or incremental growth rate and signicantly decreasing the
cost of the diet. The diet with 3% PFC obtained the best feed
conversion rate. Further, the digestibility coecients of protein
and energy are higher for levels of up to 6% PFC.
However, at increased levels of PFC, the protein eciency rate
and the productive value of protein during the fattening stage are
decreased for “green terror”.
The greater availability of PFC throughout the yr at a lower
cost compared to other oil products and raw materials such as
soybean cake, give it a comparative advantage, since it does not
have to compete with the demands of other animal species, which
in economic terms would also justify its use.
ACKNOWLEDGEMENTS
This research was supported by the Postgraduate Department
of the State Technical University of Quevedo, Ecuador and INIA-
FSE.
BIBLIOGRAPHIC REFERENCES
[1] ABIMORAD, E.; CARNEIRO, D. Métodos de colecta de
fezes e determinação dos coecientes de digestibilidade
da fração protéica e da energis dos alimentos para o pacu,
Piaractus mesopotamicus. Rev.Brasil. Zoot. 33 (1): 1101-
1109. 2004.
[2] ABRIL, V. Maracuyá, taxo y granadilla: 15 recetas nuevas en
la cocina. Facultad de Ingeniería en Alimentos, Universidad
de Cuenca. Ecuador. Tesis de Grado 30 pp. 2012.
[3] AGUIRRE, M. Evaluación de dos niveles de proteína en
dietas para juveniles de Sabaleta (Briycon henni). Rev.
Colomb. Invest. Agroindustr. 2 (1): 68-69. 2015.
[4] AMIRKOLAIE, A.; LEENHOUWERS, J.; VERRETH,
J.; SCHRAMA, J. Type of dietary ber (soluble versus
insoluble) inuences digestion, feces characteristics and
fecal waste production in Nile tilapia (Oreochromis niloticus
L.). Aquacult. Res. 36 (12): 1157-1166. 2005.
[5] ALLAN, G.; PARKINSON, S.; BOOTH, M.; STONE, D.;
ROWLAND, S.; FRANCES, J.; WARNER-SMITH, R.
Replacement of sh meal in diets for Australian silver perch,
Bidyanus bidyanus: I. Digestibility of alternative ingredients.
Aquacult. 186 (3): 327-340. 2000.
[6] ANDERSON, J.; LALL, S.; ANDERSON, D.; McNIVEN, M.
Availability of amino acids from various sh meals fed to
Atlantic salmon (Salmo salar). Aquacult. 138 (1): 291-301.
1995.
[7] BERMUDEZ, A.; MUÑOZ-RAMÍREZ, A.; WILLS, G.
Evaluación de un sistema de alimentación orgánico sobre
el desempeño productivo de la tilapia nilótica (Oreocromis
niloticus) cultivadas en estanques de tierra. Rev. Med. Vet.
Zoot. 59 (3): 165-175. 2012.
[8] BOSCOLO, W.; HAYASHI, C.; FEIDEN, A.; MEURER, F.;
SIGNOR, A. Composição química e digestibilidade aparente
da energia e nutrientes da farinha de resíduos da indústria
de letagem de tilápias, para a tilápia do Nilo (Oreochromis
niloticus). Cien. Rur. 38 (9): 2579-2586. 2010.
[9] CAVALHEIRO, O.; De SOUZA, O.; SINGH, B. Utilization
of shrimp industry waste in the formulation of tilapia
(Oreochromis niloticus Linnaeus) feed. Biores. Technol. 98
(1): 602-606. 2007.
[10] DUCHI, N.; PAZMIÑO, J. Informe nal proyecto IQ-CV-024.
Convenio de investigación ESPOCH-Promsa. Riobamba,
Ecuador. 120pp. 2002
[11] DUSTERHOFT, E.; VORAGEN, A. Non-starch and
polysaccharides from sunower (Helianthus annuus) and
palm kernel (Elaeis guineensis) meal - preparation of cell
Wall material and extraction of polysaccharide fractions. J.
Sci. Food Agricult. 55 (1): 41- 42. 1991.
[12] FOSTER, I. A note on the method of calculating digestibility
coecients of nutrients provided by single ingredients to
feeds of aquatic animals. Aquacult. Nutr. 5 (1): 143-145.
1999.
[13] FURUYA, W.; PEZZATO, L.; PEZZATO, A.; BARROS, M.;
MIRANDA, E.. Coecientes de Digestibilidade e Valores
de Aminoácidos Digestíveis de Algunos Ingredientes para
Tilápia do Nilo (Oreochromis niloticus). Rev. Brasil. Zoot.
30 (1): 1143-1149. 2001.
[14] FURUYA, M.; GONÇALVES, S.; FURUYA, B.; HAYASHI,
C. Fitase na alimentaçao da Tilapia do Nilo (Oreochromis
niloticus) desempenho e digestibilidade. Rev. Brasil. Zoot.
30: 924-929. 2001.
125
Revista Cientíca, FVC-LUZ / Vol. XXX, N° 3, 117 - 125, 2020
[15] GUIMARÃES, L.; PEZZATO, L.; BARROS, M. Amino acid
availability and protein digestibility of several protein sources
for Nile tilapia,Oreochromis niloticus. Aquacult. Nutr. 14:
396-404. 2008.
[16] HENRY-SILVA, G.; CAMARGO, M.; PEZZATO, L.
Digestibilidade aparente de macrótas aquáticas pela
tilápia-do-nilo (Oreochromis niloticus) e qualidade da água
em relação às concentrações de nutrientes. Rev. Brasil.
Zoot. 35 (1): 641-647. 2006.
[17] KÖPRÜCÜ, K.; ÖZDEMIR, Y. Apparent digestibility of
selected feed ingredients for Nile tilapia (Oreochromis
niloticus). Aquacult. 250 (3): 308-316. 2005.
[18] LUND, I.; DALSGAARD, J.; TOLDERLUND, H.; HOLM, J.;
JOKUMSEN, A.; Replacement of sh meal with a matrix
of organic plant proteins in organic trout (Oncorhynchus
mykiss) feed, and the eects on nutrient utilization and sh
performance. Aquacult. 321(1): 259-266. 2011.
[19] MADRID, J. Efecto de la sustitución en dieta de harina
de pescado con harina de productos de origen animal,
en juveniles de corvina golna, Cynoscion othonopterus.
Universidad de Baja California. México. Tesis de Grado. 56
pp. 2014.
[20] MASAGOUNDER, K.; FIRMAN, J.; HAYWARD, S.; BROWN,
P. Apparent digestibilities of common feedstus for bluegill
Lepomis macrochirus and largemouth bass Micropterus
salmoides using individual test ingredients. Aquacult. Nutr.
15 (1): 29-37. 2008.
[21] MAZÓN, C. Caracterización bromatológica de la torta de
maracuyá (Passiora edulis) procedente de los cantones de
Guayas y Vinces (Ecuador) para su uso en la alimentación
animal. Facultad de Veterinaria de la Universidad de
Córdoba. España.Tesis de Grado. 87pp. 2013.
[22] MENDOZA, R..; AGUILERA, C.; MONTEMAYOR, J.
Utilización de subproductos avícolas en las dietas para
organismos acuáticos. En: Civera-Cerecedo, R.; Pérez-
Estrada, C.J.; Ricque-Marie, D.; Cruz-Suárez, L.E. (Eds.).
Avances en Nutrición Acuícola IV. Memorias del IV
Simposium Internacional de Nutrición Acuícola. La Paz,
B.C.S. Noviembre 15-18,1998., México. Pp. 398-439. 2004.
[23] MEURER, F.; HAYASHI, C.; BOSCOLO, W. Digestibilidade
aparente de alguns alimentos protéicos pela tilápia do Nilo
(Oreochromis niloticus). Rev. Brasil. Zoot. 32 (1): 1801-
1809. 2003.
[24] NG, W.; LIM, H.; LIM, S.; IBRAHIM, C. Nutritive value of
palm kernel meal pretreated with enzyme or fermented with
Trichodemia koningii (Oudemans) as a dietary ingredient for
red hybrid tilapia (Oreochromis spp.). Aquacult. Res. 33 (1):
1199-1207. 2002.
[25] OFFICIAL METHODS OF ANALYSIS (AOAC). Animal
Feed. 18th Ed. Association of Ocial Analytical Chemists.
Gaithersburg, MD, USA. 58pp. 2005.
[26] OLIVEIRA, J.; OLIVEIRA De S, E.; SINGH, P. Utilization
of shrimp industry waste in the formulation of tilapia
(Oreochromis niloticus Linnaeus) feed. Biores. Technol.
98: 602 - 606. 2007.
[27] SAAD, C.; CHEAH, C.; KAMARUDDIN, M. Suitability
of using palm kernel cake, (PKC) in catsh practical diet.
Science and Technology Congress. Kuala Lumpur. 19
August. Malaysia. 22pp. 1994.
[28] SATOH, S.; CHO, C.; WATANABE, T. Eect of Fecal Retrieval
Timing on Digestibility of Nutrients in Rainbow Trout Diet
with the Guelph and TUF Feces Collection Systems. Nippon
Suisan Gakkaishi. 58 (6): 1123-1127. 1992.
[29] TORRES-NOVOA, D.; HURTADO, V. Requerimientos
nutricionales para Tilapia del Nilo (Oreichromis niloticus).
Orinoquia. 16 (1): 63-68. 2012.
[30] TUSCHE, K.; WUERTZ, S.; SUSENBETH, A.; SHULZ, C.
Feeding sh according to organic aquaculture guidelines
EC 710/2009: Inuence of potato protein concentrates
containing various glycoalkaloid levels on health status and
growth. Aquacult. 319 (1): 122-131. 2011.
[31] VÁSQUEZ, W.; YOSA, M.; HERNÁNDEZ, G.; GUTIÉRREZ,
M. Digestibilidad aparente de ingredientes de uso común
en la fabricación de raciones balanceadas para tilapia roja
híbrida (Oreochromis spp.). Rev. Colomb. Cien. Pec. 23
(2): 14-16. 2010.
[32] VÁSQUEZ, W.; YOSA, M.; HERNÁNDEZ, G.; GUTIÉRREZ,
M.. Digestibilidad aparente de ingredientes de origen
vegetal y animal en la cachama. Pesquisa Agrop. Brasil.
48 (8): 920-927. 2013.
[33] WAN, M.; ALIMON, A. Uso de la torta de palmiste y
subproductos de palma de aceite en concentrados para
animales. Rev. Palmas. 26 (1): 57-64. 2005.
[34] WING, K. Investigación sobre el uso de la torta de palmiste
en alimentos para la acuicultura. Rev. Palmas. 26 (3): 79-
83. 2005.
[35] YUDY, M.; LÓPEZ, O.; VÁSQUEZ, W.; ÁLVARO, F.
Evaluación de diferentes porciones de energía / proteína
en dietas para juveniles de yamú, Brycon siebenthalae
(Eigenmann, 1912). Orinoquia. 8 (1): 64 -76. 2004.
Vol, XXX, N
o
3
Esta revista fue editada en formato digital y publicada en
Diciembre 2020, por La Facultad de Ciencias Veterinarias,
Universidad del Zulia. Maracaibo-Venezuela.
www.luz.edu.ve
www.serbi.luz.edu.ve
produccioncientica.luz.edu.ve
